en Research Paper Research Paper <strong>Abstract</strong><br> Gelatin (GEL) is most extensively used in various fields, particularly in therapeutics and pharmaceuticals. GEL was extracted from goat skin using hot temperature extraction process and compared with that of commercial GEL. The physico-chemical characterization and functional properties were investigated by using temperature denaturation (T_d), water-holding and fat-binding capacities (WHC and FBC), colour measurement and UV-light spectrum. In <em>vitro</em> biocompatibility was studied for the first time and was evaluated by blood coagulation index (BCI) and haemolytic tests for using as wounds dressing. The results revealed thermal stability of goat GEL at T_d 37&deg;C. WHC and FBC capacities represented 2.5 and 1.2 g/ml, respectively. The hunter colour spaces a*, b* and L* showed a -0.27, -1.97 and 25.23 values, respectively. UV-Vis absorption spectrum of the goat GEL showed a maximum absorption peak at 280 nm. The&nbsp;<em>in vitro</em>&nbsp;anticoagulant activities of extracting GEL were higher than 70% after incubation for one hour. After being in contact with red blood cells for 1 h, the haemolysis ratio increased from to 0.46 to 1.4 when the concentration of goat GEL increased from 1 to 50 mg/ml suggesting the safety of the tested samples. These results suggest that thromboresistivity and hemocompatibility of this biopolymer retained the biological activity of our samples for biomaterial applications. According to this, goat GEL successfully competes with, and significantly could be useful for substitution of bovine in wound healing. goat gelatin,biocompatibility,natural polymer,wound dressing 1 7 http://ijmse.iust.ac.ir/browse.php?a_code=A-10-4259-2&slc_lang=en&sid=1 Salma Bessalah 1800319475328460014838 1800319475328460014838 No Livestock and Wildlife Laboratory, Arid Lands Institute (I.R.A), University of Gabès, Médenine, Tunisia Jebahi Samira jbahisamira@yahoo.fr 1800319475328460014839 1800319475328460014839 Yes Energy and Matter Research Laboratory, Centre National des Sciences et Technologies Nucleaires (CNSTN), Pole technologique, 2020, SidiThabet-Tunisia Amel Raoufi 1800319475328460014840 1800319475328460014840 No Energy and Matter Research Laboratory, Centre National des Sciences et Technologies Nucleaires (CNSTN), Pole technologique, 2020, SidiThabet-Tunisia Asim Faraz 1800319475328460014841 1800319475328460014841 No Department of Livestock and Poultry Production, BahauddinZakariya University Multan, Pakistan Mouldi Zagrouba 1800319475328460014842 1800319475328460014842 No Laboratory of physics materials, faculty of Sciences, University Of Sfax, 3000 B.P1171, Tunisia Mohamed Hammadi 1800319475328460014843 1800319475328460014843 No Livestock and Wildlife Laboratory, Arid Lands Institute (I.R.A), University of Gabès, Médenine, Tunisia